# WEB-053 — 3.5 GHz: microglial stress responses with incomplete dose reporting

Why this matters
Microglia help maintain the brain’s cellular environment. This study reported oxidative and apoptotic responses after exposure, then tested a possible protective intervention. The observations are worth locating, although incomplete absorbed-dose reporting prevents a confident comparison with environmental exposure.

Id: WEB-053

Author: Pachhapure et al.

Full Authors: Shailashree Pachhapure; Amila Mufida; Qun Wei; Jong-Soon Choi; Byeong-Churl Jang

Year: 2025

Title: Mitigation of 3.5 GHz Electromagnetic Field-Induced BV2 Microglial Cytotoxicity by Polydeoxyribonucleotide

Journal: Current issues in molecular biology

Citation: Shailashree Pachhapure; Amila Mufida; Qun Wei; Jong-Soon Choi; Byeong-Churl Jang. Mitigation of 3.5 GHz Electromagnetic Field-Induced BV2 Microglial Cytotoxicity by Polydeoxyribonucleotide. Current issues in molecular biology. 2025. doi:10.3390/cimb47060386.

Doi: 10.3390/cimb47060386

Pmid: 40699785

Topic: Microglial responses; Neuronal oxidative and apoptotic responses; 3.5 GHz cellular experiments

Verification Group: Original text reviewed

Record Role: Original text reviewed

Featured: False

Exposure: 3.5 GHz; generator setting 19 dBm; 2 h key mechanistic exposures, also 24 h. Chamber 37°C/50% RH; plates exposed with lids open.

Design: BV2 mouse microglial cells; sham controls, ROS/apoptosis assays and pathway-inhibitor/intervention comparisons.

Finding: Exposure reduced cell growth and increased DNA fragmentation, ROS and apoptosis-related signalling; PDRN attenuated several responses.

Statistics: See the original results; effect sizes were not independently recalculated.

Boundary: Generator setting is not sample SAR or incident density. No adequate absorbed-dose estimate or cell-level thermal trace was established from the source. Small mechanistic experiments; no treatment recommendation or everyday-exposure claim.

Protection Question: Which exposure conditions reproduce this finding, and what would establish its relevance beyond the experimental model?

Access: Original methods/results and interpretation reviewed; raw data and supplements were not independently audited.

Locator: Original methods and results

Notice: Linked Europe PMC/PubMed notice fields checked 21 October 2026; no correction/retraction surfaced for this record. Publisher/citation screening is not exhaustive.

Funding: Reported funder(s): Korea Basic Science Institute. Grant identifiers: A439100.

Conflicts: See original disclosure; no independently verified conflict assessment.

Source Url: https://doi.org/10.3390/cimb47060386

Retrieval Links: https://doi.org/10.3390/cimb47060386 | https://pubmed.ncbi.nlm.nih.gov/40699785/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC12192555/

Verification Date: 2026-10-21

Discovery Id: TARGETED-20261021-26

Technology Tracks: 5g

Technology Connection: Relevant carrier-frequency research; the record distinguishes actual 5G modulation from other laboratory waveforms.

Publication Type: Original journal research

Study Setting: Cells / isolated tissue

Browse Categories: brain|cells