# WEB-004 — Gene-expression and adult behaviour findings after embryonic 3.5 GHz exposure

Why this matters
A developmental response matters more when researchers follow it into later life. This follow-up examined molecular changes and adult behaviour after exposure early in development.

Id: WEB-004

Author: Dasgupta et al.

Full Authors: Subham Dasgupta; Connor Leong; Michael T Simonich; Lisa Truong; Huaping Liu; Robyn L Tanguay

Year: 2022

Title: Transcriptomic and Long-Term Behavioral Deficits Associated with Developmental 3.5 GHz Radiofrequency Radiation Exposures in Zebrafish

Journal: Environmental science & technology letters

Citation: Subham Dasgupta; Connor Leong; Michael T Simonich; Lisa Truong; Huaping Liu; Robyn L Tanguay. Transcriptomic and Long-Term Behavioral Deficits Associated with Developmental 3.5 GHz Radiofrequency Radiation Exposures in Zebrafish. Environmental science & technology letters. 2022. doi:10.1021/acs.estlett.2c00037.

Doi: 10.1021/acs.estlett.2c00037

Pmid: 35434172

Topic: Development and behaviour; 3.5 GHz developmental experiments

Verification Group: Original text reviewed

Record Role: Original text reviewed

Featured: False

Exposure: 3.5 GHz; estimated SAR 8.27 W/kg; 6–48 h after fertilisation; temperature maintained around 28°C.

Design: Zebrafish; RNA sequencing at 48 h and behavioural testing after growth to adulthood; sham exposure.

Finding: 28 differentially expressed genes, metabolic-pathway changes and subtle differences in adult behavioural assays were reported.

Statistics: See reported finding and original tables; effect sizes not independently recalculated.

Boundary: Modest effects at high estimated SAR. Linked to the earlier larval experiment; not an independent replication and not a test of a standards-specified 5G waveform.

Related: Same Oregon State programme as WEB-003; methods describe subsampling and follow-up routes.

Protection Question: Which exposure conditions and biological models reproduce this response, and what would establish its relevance to health?

Access: Original methods/results sections reviewed; no independent raw-data or supplement audit.

Locator: Original results/methods sections

Notice: Linked notice fields in retrieved Europe PMC/PubMed records checked 21 October 2026; no correction or retraction surfaced there. Publisher-history and forward-citation screening are not exhaustive.

Funding: National Institute of Environmental Health Sciences 10.13039/100000066 P30 ES030287

Conflicts: Disclosure statement not extracted in this source check; see the original publication.

Source Url: https://doi.org/10.1021/acs.estlett.2c00037

Retrieval Links: https://doi.org/10.1021/acs.estlett.2c00037 | https://pubmed.ncbi.nlm.nih.gov/35434172/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC9009179/

Verification Date: 2026-10-21

Discovery Id: EML-5G-004

Technology Tracks: 5g

Technology Connection: Relevant mobile-frequency experiment; waveform and dose described in this record.

Publication Type: Original journal research

Study Setting: Animals

Browse Categories: brain|environment