# RF-045 — Oxidative DNA-base damage in mouse spermatocyte-derived cells

Why this matters
DNA's chemical building blocks can be damaged even when a test for broken strands is negative. The abstract reports this distinction in a laboratory line of mouse reproductive cells. It points to a damage pathway for verification; full exposure controls and evidence about reproductive function remain missing.

Id: RF-045

Author: Liu et al.

Full Authors: Chuan Liu; Weixia Duan; Shangcheng Xu; Chunhai Chen; Mindi He; Lei Zhang; Zhengping Yu; Zhou Zhou

Year: 2013

Title: Exposure to 1800MHz radiofrequency electromagnetic radiation induces oxidative DNA base damage in a mouse spermatocyte-derived cell line

Journal: Toxicology Letters

Citation: Chuan Liu; Weixia Duan; Shangcheng Xu; Chunhai Chen; Mindi He; Lei Zhang; Zhengping Yu; Zhou Zhou. Exposure to 1800MHz radiofrequency electromagnetic radiation induces oxidative DNA base damage in a mouse spermatocyte-derived cell line. Toxicology Letters. 2013;218(1):2-9. doi:10.1016/j.toxlet.2013.01.003.

Doi: 10.1016/j.toxlet.2013.01.003

Pmid: 23333639

Topic: Oxidative DNA-base damage

Verification Group: Original abstract verified

Record Role: Original abstract verified

Featured: False

Exposure: 1,800 MHz GSM-talk mode,1/2/4 W/kg,24 h intermittent exposure,5 min on/10 min off.

Design: GC-2 mouse spermatocyte-derived cell line; FPG-modified comet,8-oxoG and ROS assays; α-tocopherol co-treatment.

Finding: Original abstract reports oxidative-base-damage and ROS signals at 4 W/kg, mitigated by antioxidant. Ordinary alkaline comet did not detect strand breakage.

Boundary: Full methods, replicate counts and temperature controls not retrieved. Cell line is not mature sperm or a fertility endpoint. Preserve the different assay outcomes.

Related: Chongqing Third Military Medical University programme with Xu2010; distinct from Zhejiang Xu2013.

Protection Question: Does the oxidative-base signal persist and affect reproductive-cell function? The negative ordinary comet result must remain alongside it. Full methods and temperature controls are still needed to interpret this abstract-level observation.

Access: Original PubMed abstract reviewed. Retrieval lead.

Locator: Abstract.

Notice: No editorial notice surfaced in the Scite/primary-record checks used for this record; this is not a comprehensive clean-bill certification.

Funding: Not established from retrieved material.

Conflicts: Not established from retrieved material.

Source Url: https://doi.org/10.1016/j.toxlet.2013.01.003

Retrieval Links: https://doi.org/10.1016/j.toxlet.2013.01.003 | https://pubmed.ncbi.nlm.nih.gov/23333639/

Verification Date: 2026-10-20