# RF-021 — Changed cortical gene expression after a 5G signal

Why this matters
Genes help cells respond and carry out their work; measuring which genes are active can reveal a biological response. Repeated exposure to a 5G signal changed gene activity in mouse brain tissue without altering behaviour. The health question is whether those changes have lasting consequences.

Id: RF-021

Author: Lameth et al.

Full Authors: Julie Lameth; Juliette Royer; Alexandra Martin; Corentine Marie; Délia Arnaud-Cormos; Philippe Lévêque; Roseline Poirier; Jean-Marc Edeline; Michel Mallat

Year: 2025

Title: Repeated Head Exposures to a 5G-3.5 GHz Signal Do Not Alter Behavior but Modify Intracortical Gene Expression in Adult Male Mice

Journal: international journal of molecular sciences

Citation: Lameth J, Royer J, Martin A, Marie C, Arnaud-Cormos D, Lévêque P, Poirier R, Edeline JM, Mallat M. Repeated Head Exposures to a 5G-3.5 GHz Signal Do Not Alter Behavior but Modify Intracortical Gene Expression in Adult Male Mice. International Journal of Molecular Sciences. 2025;26(6):2459. doi:10.3390/ijms26062459.

Doi: 10.3390/ijms26062459

Pmid: 40141104

Topic: Newer frequencies

Verification Group: Original text reviewed

Record Role: Original text reviewed

Featured: True

Exposure: 5G-modulated 3.5 GHz; one hour/day, five days/week; 27 exposure hours over six weeks for transcriptomics. Whole-brain average SAR 0.19 W/kg.

Design: 32 male mice overall; exposed, pseudo-exposed and unrestrained controls. Transcriptomics n=7 exposed and n=8 pseudo-exposed.

Finding: RNA sequencing detected 77 differentially expressed genes in the right sampled cortex and 84 in the left after repeated exposure, using false-discovery-rate correction.

Boundary: Behaviour was not altered. Gene-expression change is a biological response, not demonstrated brain injury. Preprint and journal paper are the same work.

Related: Distinct French research programme.

Protection Question: Do the gene-expression changes persist or alter brain function? Behaviour was unchanged. A signal-specific biological response is useful to scientific understanding without establishing injury or measuring population cumulative exposure.

Access: Full indexed article body reviewed

Locator: Results RNA sequencing; Figure 1; protocol Figure 2

Notice: Primary PubMed linked-notice record checked 20 Oct 2026; no correction, concern or retraction linked at check. This is not an exhaustive publisher-history certification.

Funding: Crossref publisher-deposited metadata lists French ANSES environmental and occupational health programme grant 2020/2 RF/14. Complete original funding/disclosure statement not rechecked.

Conflicts: Not established from retrieved material.

Source Url: https://doi.org/10.3390/ijms26062459

Retrieval Links: https://doi.org/10.3390/ijms26062459 | https://pubmed.ncbi.nlm.nih.gov/40141104/

Verification Date: 2026-10-20

Technology Tracks: 5g